Pulmonary rehabilitation protocols in urgent lung transplantation patients / 世界急诊医学杂志（英文）

@#BACKGROUND: Prolonged invasive respiratory support and extracorporeal membrane oxygenation (ECMO) in patients requiring urgent lung transplantation (ULTx) present significant challenges to clinical practice due to severe underlying diseases and complex conditions. The aim of the study was to report the clinical outcomes of patients who received ULTx and followed the perioperative rehabilitation protocol implemented in a lung transplant center. METHODS: A retrospective analysis was conducted in ULTx patients who required preoperative invasive mechanical ventilation (IMV) and ECMO between January 2018 and January 2023. Data were retrieved from electronic medical records at our lung transplant center. RESULTS: Fourteen patients (mean age 57.43±10.97 years; 12 males, 2 females) underwent ULTx with bridging ECMO and IMV. The mean body mass index was 23.94±3.33 kg/m², and the mean Acute Physiology and Chronic Health Evaluation (APACHE) II score was 21.50±3.96. The Nutritional Risk Screening 2002 (NRS 2002) scores were ≥3. ULTx was performed after an 8.5-day waiting period (interquartile interval [IQR] 5.0-26.5 d). Following the surgeries, the average lengths of ECMO and IMV were 1.0 (IQR 1.0-2.0) d and 5.0 (IQR 3.0-7.3) d, respectively. The total length of hospital stay was 60.1±30.8 d, with an average intensive care unit stay of 38.3±22.9 d and post-operative hospitalization stay of 45.8±26.1 d. Two patients died within 30 d after ULTx, with a 30-day survival rate of 85.71%. CONCLUSION: Patients receiving ULTx showed an acceptable short-term survival rate, validating the practicality and safety of the treatment protocols implemented in our center.

Strength training and coordination in soccer players' lower limbs / Treinamento da força e coordenação nos membros inferiores de futebolistas / Entrenamiento de fuerza y coordinación en miembros inferiores de futbolistas

ABSTRACT Introduction: The long distance running and the short distance running shot are common in soccer world, so that soccer presents a higher demand in relation to the strength of lower limbs and motor coordination by the athlete. Objective: Analyze the methods of training lower-limb strength and coordination in soccer. Methods: The experiment-by-control method was adopted. During the 12 weeks of training, the control group performed the traditional mode of lower limb strength training based on deep squat, while the experimental group adopted the global mode of lower limb strength training. The exercise training plans of the experimental group and control group were standardized. Results: The relative performance of the athletes in the experimental group and the control group was improved, however, the developmental effect of the experimental group was more significant. Conclusion: The comprehensive mode of lower limb strength training proposed in this study presents little difference in relation to the traditional mode of strength training in the optimization of maximum strength; however, it is more directed to soccer demand, and it can promote a better motor coordination to the athletes, besides optimizing the soccer sport level and making the athletes have more advantages in the field. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A corrida de longa distância e o tiro em corrida de curta distância são comuns no mundo do futebol, de forma que o futebol apresenta uma maior exigência em relação à força dos membros inferiores e à coordenação motora por parte do atleta. Objetivo: Analisar os métodos de treinamento de força e coordenação dos membros inferiores no futebol. Métodos: Adotou-se o método de experimento por controle. Durante as 12 semanas de treinamento, o grupo de controle efetuou o modo tradicional de treinamento da força dos membros inferiores com base no agachamento profundo, enquanto o grupo experimental adotou o modo global de treinamento da força dos membros inferiores. Os planos de treinamento de exercício do grupo experimental e do grupo de controle foram padronizados. Resultados: A performance relativa dos atletas do grupo experimental e do grupo de controle foi melhorada, contudo, o efeito de desenvolvimento do grupo experimental foi mais significativo. Conclusão: O modo abrangente de treinamento de força dos membros inferiores proposto neste trabalho apresenta pouca diferença em relação ao modo tradicional de treinamento de força na otimização da força máxima, no entanto é mais direcionado à demanda do futebol, podendo promover uma melhor coordenação motora aos atletas, além de otimizar o nível esportivo do futebol e fazer com que os atletas tenham mais vantagens em campo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La carrera de larga distancia y la carrera de corta distancia son habituales en el mundo del fútbol, por lo que el fútbol presenta una mayor exigencia en relación con la fuerza de los miembros inferiores y la coordinación motora por parte del deportista. Objetivo: Analizar los métodos de entrenamiento de la fuerza y la coordinación de los miembros inferiores en el fútbol. Métodos: Se adoptó el experimento por método de control. Durante las 12 semanas de entrenamiento, el grupo de control realizó el modo tradicional de entrenamiento de la fuerza de los miembros inferiores basado en la sentadilla profunda, mientras que el grupo experimental adoptó el modo global de entrenamiento de la fuerza de los miembros inferiores. Se estandarizaron los planes de entrenamiento físico del grupo experimental y del grupo de control. Resultados: El rendimiento relativo de los atletas del grupo experimental y del grupo de control mejoró; sin embargo, el efecto de desarrollo del grupo experimental fue más significativo. Conclusión: La modalidad integral de entrenamiento de fuerza de miembros inferiores propuesta en este trabajo presenta poca diferencia en relación a la modalidad tradicional de entrenamiento de fuerza en la optimización de la fuerza máxima, sin embargo, está más dirigida a la demanda futbolística, y puede promover una mejor coordinación motora a los atletas, además de optimizar el nivel deportivo del fútbol y hacer que los atletas tengan más ventajas en el campo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Construction of a caries diagnosis model based on microbiome novelty score / 华西口腔医学杂志

OBJECTIVES@#This study aimed to analyze the bacteria in dental caries and establish an optimized dental-ca-ries diagnosis model based on 16S ribosomal RNA (rRNA) data of oral flora.@*METHODS@#We searched the public databa-ses of microbiomes including NCBI, MG-RAST, EMBL-EBI, and QIITA and collected data involved in the relevant research on human oral microbiomes worldwide. The samples in the caries dataset (1 703) were compared with healthy ones (20 540) by using the microbial search engine (MSE) to obtain the microbiome novelty score (MNS) and construct a caries diagnosis model based on this index. Nonparametric multivariate ANOVA was used to analyze and compare the impact of different host factors on the oral flora MNS, and the model was optimized by controlling related factors. Finally, the effect of the model was evaluated by receiver operating characteristic (ROC) curve analysis.@*RESULTS@#1) The oral microbiota distribution obviously differed among people with various oral-health statuses, and the species richness and species diversity index decreased. 2) ROC curve was used to evaluate the caries data set, and the area under ROC curve was AUC=0.67. 3) Among the five hosts' factors including caries status, country, age, decayed missing filled tooth (DMFT) indices, and sampling site displayed the strongest effect on MNS of samples (P=0.001). 4) The AUC of the model was 0.87, 0.74, 0.74, and 0.75 in high caries, medium caries, low caries samples in Chinese children, and mixed dental plaque samples after controlling host factors, respectively.@*CONCLUSIONS@#The model based on the analysis of 16S rRNA data of oral flora had good diagnostic efficiency.

Application effects of feedforward control theory in the rollover bed treatment of mass patients with burn-explosion combined injury / 中华烧伤杂志

Objective: To explore the application effects of feedforward control theory in the rollover bed treatment of mass patients with burn-explosion combined injury. Methods: A retrospective observational research was conducted. From June 13 to 14, 2020, 15 patients with severe burn-explosion combined injury caused by liquefied natural gas tank car explosion and conforming to the inclusion criteria were admitted to the Second Affiliated Hospital of Zhejiang University School of Medicine. There were 13 males and 2 females, aged 33-92 (66±17) years. All the patients were treated with rollover bed from 48 h post admission, and the feedforward control theory was introduced, including establishing a special feedforward control management team for rollover bed, clarifying the duties of the medical staff in the rollover bed treatment of patients, implementing the cooperation strategy of multidisciplinary physician, training and examining for 80 nurses in the temporarily organized nurse team in the form of "rollover bed workshop", and formulating the checklist and valuation list of rollover bed treatment for continuous quality control. The frequency and the total number of turning over, and successful rate of one-time posture change with the rollover bed of patients within 30 days of admission were recorded, the occurrences of adverse events caused by improper operation for the rollover bed during the treatment were observed, including respiratory and cardiac arrests, treatment interruption, unplanned extubation, bed falling, and skin graft displacement. The lowest levels of arterial partial pressure of oxygen (PaO2) and arterial partial pressure of carbon dioxide (PaCO2), the number of patients with oxygenation index>300 mmHg (1 mmHg=0.133 kPa), and the occurrence of acute respiratory distress syndrome (ARDS) of patients within 2 days of admission and on the 30th day of admission were recorded. Results: Within 30 days of admission, the patients were turned over with the rollover bed for 2 to 6 times each day, with a total of 1 320 turning over operations, the successful rate of one-time posture change reached 99.9% (1 319/1 320), and no adverse event occurred. Within 2 days of admission, the lowest levels of PaO2 and PaCO2 of the patients were (100±19) and (42±4) mmHg, respectively, and the number of patients with mild, moderate, and severe ARDS were 10, 2, and 3, respectively, and none of the patients had oxygenation index>300 mmHg. On the 30th day of admission, the lowest levels of PaO2 and PaCO2 of the patients were (135±28) and (37±8) mmHg, respectively, 3 patients developed moderate ARDS, 1 patient developed severe ARDS, and 11 patients had oxygenation index>300 mmHg. Conclusions: The introduction of feedforward control theory in the treatment of rollover bed of mass patients with burn-explosion combined injury can ensure safe and successful completion of turning over with the rollover bed, promote the repair of burn wound, and improve respiratory function, and therefore improve the treatment quality of patients.

Practice and exploration of nursing efficiency management in a large general public hospital / 中华医院管理杂志

Under the background of efficiency medical reform, the authors introduced the nursing efficiency management system of a large public hospital, which was divided into two dimensions: nursing resource allocation efficiency and nursing service efficiency. The specific four measures included the establishment of multi-campus unified management organization structure based on nursing management committee, nursing performance reform based on structured big data information platform, the construction of nurse-led patient whole process management model and the accelerated rehabilitation nursing practice from surgery to the whole hospital, so as to provide reference for the nursing efficiency management of large general public hospitals under the medical reform.

Efficacy and safety of transapical transcatheter aortic valve replacement in the treatment of aortic regurgitation in patients with large aortic annulus / 中国胸心血管外科临床杂志

@#Objective    To investigate the feasibility and safety of transcatheter aortic valve replacement (TAVR) through apical approach for aortic regurgitation of large annulus. Methods    From November 2019 to May 2020, 10 male patients aged 64.50±4.20 years with aortic valve insufficiency (AI) underwent TAVR in the Department of Cardiovascular Surgery, Xijing Hospital. The surgical instruments were 29# J-valveTM modified and the patients underwent TAVR under angiography. The preoperative and postoperative cardiac function, valve regurgitation, complications and left ventricular remodeling were summarized by ultrasound and CT before and after TAVR. Results    A total of 10 valves were implanted in 10 patients. Among them, 1 patient was transferred to the aortic arch during the operation and was transferred to surgical aortic valve replacement; the other 9 patients were successfully implanted with J-valve, with 6 patients of cardiac function (NYHA) class Ⅱ, 4 patients of grade Ⅲ. And there was a significant difference between preoperation and postoperation in left ventricular ejection fraction (44.70%±8.78% vs. 39.80%±8.48%, P<0.05) or aortic regurgitation (1.75±0.72 mL vs. 16.51±8.71 mL, P<0.05). After 3 months, the patients' cardiac function was good. Conclusion    TAVR is safe and effective in the treatment of severe valvular disease with AI using J-valve.

Analysis on comparison of inter-laboratory results of chemical volatile organic compounds / 中国职业医学

OBJECTIVE: To analyze the comparison results of volatile organic components in chemicals tested by occupational health laboratories.METHODS: A total of 37 reference laboratories that participated in the 2019 National Occupational Health Inspection and Testing Institution Laboratory Comparison Chemical Qualitative Testing Comparison organized by Guangdong Occupational Health Testing Center were selected as the research subjects. Headspace gas chromatography-mass spectrometry was used for the determination of volatile organic components in chemicals. The comparison results of reference laboratories were collected and implemented with qualitative and quantitative evaluation. RESULTS: The qualified rates of the qualitative results of the required hazard factors and other hazard factors in the reference laboratories were higher than those of the quantitative results of similar factors with statistical significance(83.78% vs 67.57%, 89.19% vs 56.76%, all P<0.05). There was no significant difference in the qualified rate of qualitative and quantitative results and comprehensive judgment results among each reference laboratory with other hazard factors(83.78% vs 89.19%, 67.57% vs 56.76%, 83.78% vs 89.19%, all P>0.05). The qualified rate of 37 reference laboratories was 89.19%(33/37). It showed no significant difference in the qualified rate of qualitative, quantitative and comprehensive judgment results among the reference laboratories of disease prevention and control system and non-disease prevention and control system(93.75% vs 85.72%, 85.00% vs 61.91%, 93.75% vs 85.52%,all P>0.05). CONCLUSION: There are great differences in the detection ability of volatile organic components on chemicals of each reference laboratory. The ability of qualitative detection is superior to the quantitative detection.

Design and Application of Intelligent Management System of Artificial Airway Airbag Pressure in Intensive Care / 中国医疗器械杂志

In order to solve the problem of continuous monitoring and automatic regulation of patient airbag pressure in intensive care unit, the study designed an intelligent management system of artificial airway airbag pressure. It can realize real-time monitoring and automatic control of airbag pressure. Its pressure data was sent to the PC in real time by the serial port. It can realize the display, store, review and analysis of pressure data. Its clinical application effect was discussed. Experiments showed that the system can monitor airbag pressure in real time and control the pressure to stabilize at 25~30 cmH

Mechanism of Xiaojinwan Against Breast Cancer Bone Metastasis： An Exploration Based on Bioinformatic Analysis Combined with Cell Experiments / 中国实验方剂学杂志

Objective:To explore the mechanism of Xiaojinwan in treating breast cancer bone metastases through cell experiments and bioinformatic analysis. Method:The inhibitory effect of Xiaojinwan on MCF-7 cell viability was detected by cell counting kit-8 （CCK-8） assay. The key components and targets responsible for Xiaojinwan in inhibiting breast cancer bone metastases were predicted by network pharmacology and molecular docking. The active components and targets of Xiaojinwan were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCSMP） and SwissTarget Prediction， and the breast cancer bone metastases-related targets from GeneCards and DisGeNET. The results were imported into STRING for constructing a protein-protein interaction （PPI） network， followed by Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis using DAVID. A network of the active components of Xiaojinwan-breast cancer bone metastases-related targets-pathways was constructed using Cytoscape 3.7.2. AutoDock 4 was employed for molecular docking. The protein expression levels of matrix metallopmteinase-9 （MMP-9）， hypoxia-inducible factor 1<italic>α </italic>（HIF1A）， and androgen receptor （AR） were assayed by Western blot. Result:Xiaojinwan inhibited the viability of MCF-7 cells and acted on breast cancer bone metastases through such processes as redox and protein autophosphorylation. KEGG enrichment analysis showed that HIF-1， vascular endothelial growth factor （VEGF） and phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） pathways were involved. As verified by molecular docking， the active components such as eucalyptin stably bound to AR and MMP-9. Western blot indicated that Xiaojinwan dose-dependently inhibited the expression of MMP-9 and HIF1A proteins in MCF-7 cells. Conclusion:Xiaojinwan acts on AR and MMP-9 through HIF， VEGF and other related signaling pathways， thereby improving hypoxia in tumor microenvironment， inhibiting angiogenesis， and reducing cell invasion and viability.

Therapeutic effect of robot-assisted laparoscopic sacrocolpopexy in the treatment of pelvic organ prolapse / 中南大学学报(医学版)

OBJECTIVES@#To assess short-term functional outcomes achieved by robot-assisted sacrocolpopexy for pelvic organ prolapse.@*METHODS@#We retrospectively collected clinical and operative data for female patients who underwent either pure laparoscopic sacrocolpopexy (a control group, =20) or robot-assisted laparoscopic sacrocolpopexy (a study group, =20) between December 2017 and December 2018. The clinical indicators included age, gestational age, parity, the stage of pelvic organ prolapse. Perioperative data included operative time and total blood loss. Post-operative outcomes included hospital stay, the time of detaining urethral catheterization, and the restart of anal exhaust after surgery. At the same time, complications and quality of life were observed till 6 months after the surgery.@*RESULTS@#There were no definitely differences in the perioperative data between the 2 groups. It is worth mentioning that robot-assisted laparoscopic sacrocolpopexy was superior in strict operative time. With a follow-up of 6 months, the study group's anatomic repair rate was 100% (20/20), while the control group was 95% (19/20). Pelvic Floor Distress Inventory-short Form 20 (PFDI-20) and Pelvic Floor Impact Questionnaire-short Form 7 (PFIQ-7) were used to evaluate patients' quality of life. There was no significant difference in the scores between the study group and the control group before and 6 months after surgery. Only the data of the PFDI-20 questionnaires at 1 month after operation were statistically significant, and in the control group was larger than that in the study group, showing that robotic surgery can recover faster than laparoscopy, and the quality of life can be improved quickly.@*CONCLUSIONS@#Robot-assisted laparoscopic sacrocolpopexy is a safe and reliable technique, faster than laparoscopy in recovery and has a short-term effect.

Nursing care of a case of cardiogenic shock complicated with Tako-Tsubo cardiomyopathy treated by extracorporeal membrane pulmonary oxygen / 中国实用护理杂志

Objective@#To introduce the nursing care of a case of Tako-Tsubo cardiomyopathy treated with extracorporeal membrane oxygenation (ECMO) and to improve the understanding of Tako-Tsubo cardiomyopathy and ECMO.@*Methods@#ECMO nursing group combined with medical treatment group was established to treat Tako-Tsubo cardiomyopathy patients with ECMO, special nursing, division of labor, close monitoring of vital signs and related indicators of Tako-Tsubo cardiomyopathy, ECMO nursing and prevention of complications.@*Results@#ECMO on board for 30 minutes, blood gas analysis was improved, the relative indexes of Tako-Tsubo cardiomyopathy were gradually improved after ECMO, the left ventricular ejection fraction gradually recovered after 2 days, and the wall motion gradually returned to normal. After 3 days, the value of serological biomarkers decreased obviously. After 4 days, ECMO therapy was stopped and ECMO tube was removed. After 5 days, tracheal intubation was removed. The patient was stable in condition and discharged from hospital after 41 days of inpatient treatment.@*Conclusions@#A rapid and effective ECMO nursing team is an important guarantee for the successful treatment of patients. Close observation of the condition, good team cooperation and reasonable division of labor can be effective. To improve the prognosis of patients.

Nursing care of a case of cardiogenic shock complicated with Tako-Tsubo cardiomyopathy treated by extracorporeal membrane pulmonary oxygen / 中国实用护理杂志

Objective To introduce the nursing care of a case of Tako-Tsubo cardiomyopathy treated with extracorporeal membrane oxygenation (ECMO) and to improve the understanding of Tako-Tsubo cardiomyopathy and ECMO. Methods ECMO nursing group combined with medical treatment group was established to treat Tako-Tsubo cardiomyopathy patients with ECMO, special nursing, division of labor, close monitoring of vital signs and related indicators of Tako-Tsubo cardiomyopathy, ECMO nursing and prevention of complications. Results ECMO on board for 30 minutes, blood gas analysis was improved, the relative indexes of Tako-Tsubo cardiomyopathy were gradually improved after ECMO, the left ventricular ejection fraction gradually recovered after 2 days, and the wall motion gradually returned to normal. After 3 days, the value of serological biomarkers decreased obviously. After 4 days, ECMO therapy was stopped and ECMO tube was removed. After 5 days, tracheal intubation was removed. The patient was stable in condition and discharged from hospital after 41 days of inpatient treatment. Conclusions A rapid and effective ECMO nursing team is an important guarantee for the successful treatment of patients. Close observation of the condition, good team cooperation and reasonable division of labor can be effective. To improve the prognosis of patients.

Clinical analysis for 156 cases of vaginoscopy in young girls and adolescent girls / 中南大学学报(医学版)

To investigate the clinical value of vaginal endoscopy in the diagnosis and treatment for vaginal and uterine diseases in young girls and adolescent girls. 
 Methods: The clinical data of 156 young girls and adolescent girls treated with vaginoscopy from February 2000 to August 2017 were analyzed retrospectively.
 Results: Of the 37 cases of young girls, the clinical symptoms were the most common in vaginal hemorrhage, accounting for 40.5% (15 cases), the second one in vaginal foreign body, accounting for 29.7% (11 cases), the third one in abnormal vaginal secretion, accounting for 24.3% (9 cases), and the last one in low abdominal pain, accounting for 5.4% (2 cases). The final diagnosis of vaginal foreign body was 13 cases (35.1%). Of the 119 adolescent girls, the clinical symptoms of the visit were the most common in menstrual abnormalities or irregular vaginal bleeding, accounting for 75.6% (90 cases). The final diagnosis of abnormal uterine bleeding-ovulatory dysfunction was the most common with 59 cases (49.6%), following by 16 cases of abnormal uterine bleeding-polyp, 2 cases of abnormal uterine bleeding-malignancy and hyperplasia, and 1 cases of abnormal uterine bleeding-coagulopathy. There were 37 cases (31.1%) of reproductive tract malformation. All 156 patients successfully completed vaginal endoscopy and operation with only 1 case of urinary system infection. No other complications occurred and no damage in the hymen. 
 Conclusion: Vaginal endoscopy is a safe, effective and near-noninvasive way for the diagnosis and treatment of vaginal or uterine diseases in young girls and adolescent girls. In the case of abnormal vaginal secretions and vaginal bleeding, it should be properly recommended to use the vaginal endoscopy for diagnosis and treatment.

Clinical characteristics and postoperative complications of elderly patients with biliary pancreatic disease treated with ERCP / 中国内镜杂志

Objective To investigate the clinical characteristics and postoperative complications of elderly patients with biliary pancreatic disease treated with endoscopic retrograde cholangiopancreatogra. Methods The clinical data of 236 patients with ERCP were analyzed retrospectively. According to the age of over 80 years or not, the patients were divided into the elderly group and non-elderly group, and the clinical characteristics and complications of elderly patients were explored. Results In 116 cases of elderly patients, the common bile duct stones also were the primary disease, but the proportion of malignant obstructive jaundice was significantly increased. Compared with the non-elderly patients, the incidence of hypertension, coronary heart disease, diabetes mellitus, COPD and duodenal papillary diverticulum were increased significantly in the elderly group. Moreover, the proportion of biliary stent implantation were increased significantly. The incidence of postoperative bleeding in the elderly patients were significantly higher than that in the non-elderly patients, and the high risk factors might be primary malignant obstructive jaundice, with hypertension, coronary heart disease and duodenal papillary diverticulum. Conclusion The prevalence of malignant diseases in elderly patients was increased, and they have a lot of complications, such as hypertension, coronary heart disease, diabetes mellitus, COPD and duodenal papillary diverticulum, which resulting in increased risk of postoperative bleeding. In general, therapeutic ERCP was safe and effective for elderly patients with biliary pancreatic diseases.

Expressions of GPR81, MCT1 and MCT4 in squamous carcinoma and their clinical significance / 中南大学学报(医学版)

To validate the expressions of G protein-coupled receptor 81 (GPR81), monocarboxylate transporter (MCT) 1 and MCT4 in cervical squamous carcinoma and to explore their role in the onset of cervical squamous carcinoma.
 Methods: Immunohistochemical method was used to detect the expressions of GPR81, MCT1 and MCT4 in 16 normal cervical tissue and 44 cervical squamous carcinoma tissue. The associations of these proteins expression with cervical squamous carcinoma or clinicopathological factors were analyzed.
 Results: The expressions of GPR81, MCT1 and MCT4 in cervical squamous carcinoma tissue were higher than those in normal cervical tissue (P0.05). No difference of the expressions of GPR81, MCT1 and MCT4 were found between cases with or without lymphatic metastasis (P>0.05). No correlation was found among GPR81, MCT1 and MCT4 in cervical squamous carcinoma (P>0.05).
 Conclusion: GPR81, MCT1 and MCT4 may be associated with the onset of cervical squamous carcinoma, and GPR81 may be associated with the progression of cervical squamous carcinoma.

Effect of intracerebellar microinjection of cdc2-siRNA on the coordination of mice with Niemann-Pick disease type C / 中华行为医学与脑科学杂志

Objective To observe the effect of inhibiting the abnormal activation of cdc2 gene on the coordination of mice with Niemann-Pick disease type C(NPC).Methods Recombinant adeno-associated virus(rAAV) encoding cdc2-siRNA was packaged,and then was injected into the cerebellum of 2 weeks old npc-/-mice.Footprint test and vertical screen test were performed to assess the coordination of mice at the age of 8 weeks.Purkinje cells visualized by HE staining in cerebellum were counted,and the phosphorylation of microtubule-associated protein Tau recognized by PHF-1 antibody was detected by immunoblotting technology.Results (1) Footprint test showed that the stride length in cdc2-siRNA npc-/-group((4.92±0.31)cm) was markedly longer than that in empty vector npc-/-group((4.05 ± 0.19) cm) (P＜ 0.05).(2) Vertical screen test showed that the latency to turn head upwards or reach the upper edge of the screen in cdc2-siRNA npc-/-group((26.01± 1.82) s,(50.93±1.98) s) was significantly shorter than that in the empty vector npc-/-group ((31.96± 3.47) s,(56.89 ± 2.97) s),respectively (P＜ 0.05 for all comparisons).(3) The number of Purkinje cells in cerebellum was dramatically increased in cdc2-siRNA npc-/-group(11.0±2.5) compared with the empty vector npc-/-group (5.1 ± 2.2) (P＜0.05).(4)The relative optical densities of cdc2 and phosphorylated Tau immunoreactive bands in cdc2-siRNA npc-/-group(1.42±0.22,0.95±0.31)were significantly lower than those in the empty vector npc-/-group(2.11±0.29,2.61±0.62),respectively (P＜0.05 for all comparisons).Conclusion Inhibiting the abnormal activation of cdc2 gene can improve the coordination of npc-/-mice by ameliorating Purkinje cell's loss and reducing the hyperphosphorylation of Tau in cerebellum.

Preparation and characterization of an injectable bioactive calcium phosphate material for bone repair / 中国组织工程研究

BACKGROUND:In view of the unavoidable problems of autogenous and al ogenous bone grafts, it is an urgent need to develop biodegradable bone substitute materials, among which is calcium phosphate material that has become a hot spot in the domestic and foreign research. OBJECTIVE:To develop a biodegradable calcium phosphate material for bone repair based on tetracalcium phosphate (TTCP). METHODS:The biodegradable calcium phosphate cement made from TTCP, dicalcium phosphate anhydrous and different constituents of curing liquids was prepared under room temperature (about 25 ℃). The effects of solid components, liquid components as well as calcinations and drying temperature on the physical and biological performances were detected through X-ray diffraction test, hardness test, decay in a simulated body fluid, hemolysis and cytotoxicity tests, respectively, to select the bone repair material with excel ent performances. RESULTS AND CONCLUSION:When the calcination temperature was lower than 1300 ℃, TTCP was rarely available;only close to 1400 ℃, the relatively pure TTCP was gained. A large number of pure TTCP were gained by rapid cooling because of avoidance of the moisture impact, but slow cooling made the main products to be hydroxyapatite, suggesting that rapid cooling is essential to obtain pure TTCP. With the increase of the proportion of citric acid solution in the liquid phase, the pH values and the hemolysis rate in the bone cement soak solution were increased gradually, illustrating that citric acid solution is easy to induce hemolysis. In vitro cel experiments showed that the hemolysis rate of bone cement with a solution of 2:1 NH4/Na ratio was the lowest, and the biocompatibility was the best, which was the most favorable to cel growth. Cements was made of calcined powders sieved at 1400 ℃ and showed the shortest initial setting time, least effect on pH values, lowest disintegration rate and hemolysis rate, and slightest inhibition effect on the cel proliferation, indicating that the bone cements made of sieved powder after 1400 ℃ calcination is more suitable for clinical application.

A case report for primary yolk sac tumor of endometrium / 中南大学学报(医学版)

A case of primary yolk sac tumor of endometrium was retrospectively analyzed and relevant literature was systematically reviewed. We found that the primary yolk sac tumor of endometrium showed low incidence rate and was clinically characterized by abnormal vaginal bleeding, intrauterine lesions and significantly elevated alpha-fetoprotein (AFP) level. The final diagnosis should be confirmed by pathological examination. There is no guideline for the treatment of primary yolk sac tumor of endometrium due to its rareness. For now, surgery and chemotherapy are the major therapies and the curative effect is satisfactory in some cases.

Van Wyk and Grumbach syndrome:A case report and literature review / 吉林大学学报(医学版)

Objective:To analyze the clinical materials of the patient with Van Wyk and Grumbach syndrome (VWGS),and to explore the pathogenesis,clinical characteristics,diagnosis,differential diagnosis and treatment method.Methods:The clinical data of a patient with VWGS was analyzed retrospectively.Results:The patient presented autoimmune thyroiditis, sever long-standing hypothyroidism, bilateral ovarian cysts, hyperprolactinaemia and a secondary pituitary adenoma. After thyroxine replacement treatment, the thyroid function and prolactin (PRL)level recovered to the normal levels and the ovarian cyst was shrunk within 1 month. Conclusion:The clinical manifestation of VWGS is special.And it’s easy to misdiagnose this syndrome as pituitary adenoma or ovary cyst and then operation is performed.Thyroid hormone replacement can completely resolve the symptoms and hormone abnormalities.Grasping the clinical characteristics of this syndrome and making correct diagnosis is critical to avoid unnecessary operation.

Expressions of ASPP2, iASPP and p53 in cervical cancinoma and the significance / 中南大学学报(医学版)

OBJECTIVE@#To explore the role of apoptosis stimulating proteins of p53 2 (ASPP2) and inhibitory member of the ASPP family (iASPP) in the occurrence of cervical cancer and the relation between these two proteins and p53.@*METHODS@#We used immunohistochemical method to detect the expression of ASPP2, iASPP, p53 in 51 patients with early cervical cancer tissue, 53 patients with cervical intraepithelial neoplasia (CIN) II-III, 48 patients with CIN I and 45 patients with normal cervical tissue. The relation among ASPP2, iASPP and p53 was analyzed.@*RESULTS@#When p53 was negative, the positive expression rate of ASPP2 in the cervical cancer group, the CIN II-III group, the CIN I group and the normal cervix group was gradually increased. There was significant difference between the CIN II-III group or the cervical cancer group and the normal cervix (P0.05). The positive expression rate of iASPP in the 4 groups gradually reduced, and the difference was significant difference between the cervical cancer group or the CIN II-III group and the normal cervix group (P0.05).@*CONCLUSION@#ASPP2 and iASPP may play an important role in the occurrence of early stage of cervical cancer by regulating the ability of wild type p53 in induction of apoptosis. ASPP2 and iASPP gene might be a potential molecular target for cervical carcinoma.